1.
J Synchrotron Radiat
; 29(Pt 6): 1429-1435, 2022 Nov 01.
Artigo
em Inglês
| MEDLINE
| ID: mdl-36345751
RESUMO
Brownian motion of Cowpea mosaic virus (CPMV) in water was measured using small-angle X-ray photon correlation spectroscopy (SA-XPCS) at 19.2â µs time resolution. It was found that the decorrelation time τ(Q) = 1/DQ2 up to Q = 0.091 nm-1. The hydrodynamic radius RH determined from XPCS using Stokes-Einstein diffusion D = kT/(6πηRH) is 43% larger than the geometric radius R0 determined from SAXS in the 0.007â M K3PO4 buffer solution, whereas it is 80% larger for CPMV in 0.5â M NaCl and 104% larger in 0.5â M (NH4)2SO4, a possible effect of aggregation as well as slight variation of the structures of the capsid resulting from the salt-protein interactions.